| Place | Time | Relay Swimmerws (Yrs) | School | City | State |
| 1 | 3:21.20 | Veronica Burchill (10), Katie Lemen (12), Kendall Smith (10), Claire Adams (10) | Carmel High School | Carmel | IN |
| 2 | 3:21.34 | Kaley Buchanan (9 ), Vivian Tafuto (11), Madelyn Veith (12), Meaghan Raab (12) | Hershey High School | Hershey | PA |
| 3 | 3:21.41 | Ella Eastin (11), Emily Eastin (10), Tianna Jorgenson (10), Lindsey Engel (12) | Crean Lutheran High School | Irvine | CA |
| 4 | 3:22.03 | Racheal Bukowski (12), Aly Tetzloff (11), Hannah Kukurugya (9 ), Mackenzie Artim (10) | Crown Point High School | Crown Point | IN |
| 5 | 3:22.15 | Nora McCullagh (11), Elsa Welshofer (12), Rebecca Postoll (11), Lauren Rhodes (12) | Charlotte Catholic High School | Charlotte | NC |
| 6 | 3:22.50 | Abby Jagdfeld (12), Molly Manchon (11), Kaersten Meitz (12), Kierston Farley-Sepe (10) | Waukesha South High School | Waukesha | WI |
| 7 | 3:22.79 | Asia Seidt (10), Ashley LeClair (12), Leah Stevens (11), Brooke Bauer (10) | Sacred Heart Academy | Louisville | KY |
| 8 | 3:23.07 | Emily Allen (11), Heather Merritt (12), Rachel Bright (11), Jessica Merritt (10) | William A. Hough High School | Cornelius | NC |
| 9 | 3:23.70 | Kasey Schmidt (11), Claire Rasmus (11), Natasha Gvakharia Mgebrishvili (11), Jessica Hodgson (12) | The Bolles School | Jacksonville | FL |
| 10 | 3:23.77 | Kelly Jacob (10), Megan Doty (11), Sarah Laabs (10), Mary Warren (12) | Arrowhead High School | Hartland | WI |
| 11 | 3:24.32 | Elizabeth Devitt (12), Sydney Franzen (11), Emily Boggess (10), Kathryn McLaughlin (11) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 12 | 3:24.33 | Kylie Mihok (10), Gretta DeCoursey (9 ), Taite Kitchel (11), Alex Cleveland (12) | Zionsville Community High School | Zionsville | IN |
| 13 | 3:25.03 | Selby Sturzenegger (12), Kayla Holman (10), Kathryn Bower (9 ), Ally Howe (12) | Sacred Heart Preparatory | Atherton | CA |
| 14 | 3:25.16 | Emily Albrecht (11), Lindsay Mathys (9 ), Maddy Sims (12), Gabby Sims (12) | Downers Grove North High School | Downers Grove | IL |
| 15 | 3:25.17 | Megan Moroney (11), Rachael Bradford-Feldman (11), McKenna Keith (11), Brenna Ruth (12) | Saint Andrew's School | Boca Raton | FL |
| 16 | 3:25.37 | Madelyn Murphy (10), Kelsey Witkay (10), Samantha Coloma (10), Natalie Amberg (12) | Carondelet High School | Concord | CA |
| 17 | 3:25.44 | Kaitlyn Albertoli (11), Brittany Kahn (11), Katie Glavinovich (9 ), Karli Thuen (11) | Jserra Catholic High School | San Juan Capistrano | CA |
| 18 | 3:25.48 | Sarah Kaunitz (12), Sayeh Faridnia (11), Lisa Kaunitz (10), Carly Reid (12) | Monta Vista High School | Cupertino | CA |
| 19 | 3:25.56 | Erin Sheehan (10), Grace Van Fossen (11), Jennifer Smith (12), GraceAnne Long (11) | Upper Arlington High School | Upper Arlington | OH |
| 20 | 3:25.69 | Kaia Grobe (11), Shelby Holmes (12), Kylie Dahlgren (11), Zoe Avestruz (11) | Chanhassen High School | Chanhassen | MN |
| 21 | 3:26.24 | Maggie Jahns (10), Abbey Schneider (11), Emily Moser (11), Lauryn Parrish (9 ) | Fishers High School | Fishers | IN |
| 22 | 3:26.41 | Jacquelyn Du (11), Lauren Mcgrath (9 ), Kayla Churman (11), Morgan Soulia (11) | North Allegheny Senior High School | Wexford | PA |
| 23 | 3:26.50 | Pauline Kaminski (12), Catie Wallace (10), Maggie Sheean (12), Mimi Schneider (12) | Fenwick High School | Oak Park | IL |
| 24 | 3:26.61 | Erin Falconer (11), Jennifer Kordik (11), Rachel Robb (11), Karrie Kozokar (12) | Mundelein High School | Mundelein | IL |
| 25 | 3:26.77 | Alexandra Rieger (12), Heather Johnson (12), Emma Barksdale (11), Chenoa Devine (10) | Davis Senior High School | Davis | CA |
| 26 | 3:26.79 | Laura Branton (11), Kendall Lawhorn (12), Megan Byrnes (10), Janet Hu (12) | Oakton High School | Vienna | VA |
| 27 | 3:26.98 | Dina Rommel (12), Rebecca Anthone (12), Victoria Butler (12), Sydney Modeas (12) | Clarence High School | Clarence | NY |
| 28 | 3:27.30 | Rachel Thompson (12), Brooke Maxson (10), Courtney Mykkanen (10), Emily Konishi (11) | Foothill High School | Santa Ana | CA |
| 29 | 3:27.52 | Emma Cleason (9 ), Shannon Cowley (12), Kaelan Oldani (10), Katie Portz (10) | Skyline High School | Ann Arbor | MI |
| 30 | 3:27.56 | Alexandra Aitchison (11), Madison Kennedy (12), Michelle Turek (11), Sydney Pickrem (11) | East Lake High School | Tarpon Springs | FL |
| 31 | 3:27.74 | Samantha Smith (9 ), Emily Protz (10), Lindsay Painton (10), Taylor Wilson (11) | Regis Jesuit High School | Aurora | CO |
| 32 | 3:27.79 | Holly Morren (12), Cassie Misiewicz (12), Emily John (12), Taylor Garcia (11) | Holland High School | Holland | MI |
| 33 | 3:27.88 | Amelia Girgenti (12), Brittany Bishop (11), Nicole Retondo (11), Ingrid Wall (9 ) | New Trier High School | Winnetka | IL |
| 34 | 3:27.98 | Spence Atkins (10), Nancy Hu (11), Laura Toth (12), Niamh O'Grady (12) | Riverview High School | Sarasota | FL |
| 35 | 3:27.99 | Rachel Smith (10), Marina Smith (12), Alexa Fusari (9 ), Claire Therien (10) | San Ramon Valley High School | Danville | CA |
| 36 | 3:28.02 | Daria Pyshnenko (9 ), Haley Nelson (10), Caitlin Lanigan (12), Reilly Lanigan (10) | Lake Forest High School | Lake Forest | IL |
| 37 | 3:28.07 | Colleen Donlin (10), Carly Quast (9 ), Sarah Lingen (10), Anna Petty (10) | Wayzata High School | Plymouth | MN |
| 38 | 3:28.16 | Jessie Li (11), Michaela Mullison (10), Brittney Beetcher (9 ), Julia Box (11) | Fairview High School | Boulder | CO |
| 39 | 3:28.17 | Jennifer Campbell (11), Gabrielle Bethke (12), Fiona Hon (12), Nia Gardner (11) | Gunn High School | Palo Alto | CA |
| 40 | 3:28.34 | Kathleen Hanley (9 ), Reagan Roob (9 ), Hope Christy (9 ), Rachel Hayden (12) | North Central High School | Indianapolis | IN |
| 41 | 3:28.36 | Alicia Tiberino (12), Emily Andrews (12), Elaina Gu (11), Hannah Lindsey (10) | Winston Churchill High School | Potomac | MD |
| 42 | 3:28.53 | Vanessa Krause (10), Andrea Hanas (9 ), Amy Socha (9 ), Kelly Craig (10) | Chesterton High School | Chesterton | IN |
| 43 | 3:28.59 | Hannah Grubbs (12), Mary Ashby (11), Allison Stevens (12), Emily Ward (10) | Campolindo High School | Moraga | CA |
| 44 | 3:28.60 | Tamara Santoyo (11), Alyssa Schultz (10), Emma Osowski (12), Ranslem Mikayla (11) | William S. Hart High School | Newhall | CA |
| 45 | 3:28.61 | Casey Rose (11), Rebecca Upton (12), Alexa Baran (12), Emily Gibson (12) | Southlake Carroll High School | Southlake | TX |
| 46 | 3:28.66 | Kiley Eble (11), Agnes Mirando (12), Abby Koerwitz (10), Macie McNichols (12) | Gilmour Academy | Gates Mills | OH |
| 46 | 3:28.66 | Amelia Armstrong-Grant (10), Serena Yan (12), Allison Eppinga (10), Kate Rogers (12) | Saline Area Schools | Saline | MI |
| 48 | 3:28.68 | Samantha Howell (9 ), Annalisa Parker (11), Caitlyn Plattel (10), Iris Brand (11) | Amador Valley High School | Pleasanton | CA |
| 48 | 3:28.68 | Maria Kyle (10), Maria Jardeleza (11), Olivia Andrew (9 ), Grace Tierney (11) | Loyola Academy | Wilmette | IL |
| 50 | 3:28.75 | Emma Cole (9 ), Gabbie Gauntt (9 ), Mary Beckwith (11), Kira de Bruyn (12) | Walton High School | Marietta | GA |
| 51 | 3:28.91 | Kayla Owens (12), Gina DeFrancesco (12), Megan Douds (10), Monica Sowinski (11) | Greater Latrobe High School | Latrobe | PA |
| 51 | 3:28.91 | Natalie Tapia (10), Marysol Arce (11), Rebeca Martinez (12), Nikol Lagodzinska (12) | St. Ignatius College Prep | Chicago | IL |
| 53 | 3:28.99 | Kailyn Evans (9 ), Erin O'Neill (10), Aimee Baur (10), Tara Rogers (12) | North Penn High School | Lansdale | PA |
| 54 | 3:29.11 | Mary Beth Blocher (12), Megan Wimmer (9 ), Allison Cunningham (11), Nicole Smith (11) | Munster High School | Munster | IN |
| 55 | 3:29.22 | Ashley Pollok (9 ), Kelsey Opiela (11), Aley Shimel (9 ), Lexie Lupton (11) | Kingwood High School | Kingwood | TX |
| 56 | 3:29.27 | Mykenzie Leehy (9 ), Emswiler Harper (12), Lindsay Keltch (11), Lauren Leehy (12) | Lewis Central High School | Council Bluffs | IA |
| 57 | 3:29.33 | Caitlin Cooper (12), Joelle Borchers (11), Sarah Coats (12), Kathleen Cook (12) | Woodward Academy | College Park | GA |
| 58 | 3:29.36 | Katherine Sweetser (9 ), Danielle Liu (12), Kaitlin Simpson (12), Amy Zhao (12) | Phillips Academy | Andover | MA |
| 59 | 3:29.50 | Julieyanna Parker (10), Stephanie Mauer (10), Megan Bull (9 ), Lindsay Temple (12) | Hillsborough High School | Hillsborough | NJ |
| 60 | 3:29.54 | Caroline Gaertner (11), Lorelei Gaertner (9 ), Emily Conners (10), Abbie Dolan (10) | Visitation High School | Mendota Heights | MN |
| 61 | 3:29.60 | Taylor Newton (10), Nora Deleske (9 ), Delaney Cleveland (11), Devin Newton (12) | Edison High School | Huntington Beach | CA |
| 62 | 3:29.71 | Kylie Sutherlin (10), Allie Barnes (10), Robyn Clevenger (10), Natalie Mudd (10) | Hamilton Southeastern High School | Fishers | IN |
| 63 | 3:29.83 | Lizzie Troy (11), Zoe Thatcher (12), Abbey Esler (10), Jordan Decker (10) | Mason High School | Mason | OH |
| 64 | 3:29.86 | Tru Walor (10), Delaney Ambrosen (11), Anna Justinak (10), Isabel Wyer (12) | Minnetonka High School | Minnetonka | MN |
| 65 | 3:29.93 | Lauren Cuda (12), Elizabeth Ryan (10), Ellery Parish (9 ), Anelise Diener (11) | Alamo Heights High School | San Antonio, Texas | TX |
| 66 | 3:29.95 | Sara Metzsch (10), Riley Neal (9 ), Logan Neal (11), Ellie Thornbrue (11) | Century High School | Hillsboro | OR |
| 67 | 3:30.08 | Sarah Medellin (12), Tori Kroon (12), Erin Sutton (11), Kathryn Carson (12) | Cinco Ranch High School | Katy | TX |
| 68 | 3:30.09 | Emily Cornell (10), Maura Mcmanus (9 ), Olivia Giampietro (9 ), Julie Dobson (11) | Villa Maria Academy | Malvern | PA |
| 69 | 3:30.12 | Grace Herron (11), Katie Garrity (10), Olivia Johnston (11), Megan Wenman (10) | Rosemount High School | Rosemount | MN |
| 70 | 3:30.22 | Samantha Giannetta (11), Sarah Snyder (10), Skylar Elkington (9 ), Mary Brooks (12) | Clovis West High School | Fresno | CA |
| 70 | 3:30.22 | Haley Fisher (11), Megan Hawk (12), Morgan Waggoner (10), Meghan Moses (10) | Fremont Ross High School | Fremont | OH |
| 70 | 3:30.22 | Miriyam Ghali (11), Julia Chui (10), Tyla Martin (11), Hannah Veale (12) | Pine Crest School | Fort Lauderdale | FL |
| 73 | 3:30.23 | Kathryn Nicholson (9 ), Katie Ford (12), Meghan Schilken (12), Hannah Bumgarner (10) | Mt. Lebanon High School | Pittsburgh | PA |
| 73 | 3:30.23 | Hanna M. Gresser (9 ), Samantha J. Geyer (11), Carly C. Hart (9 ), Lauren E. Heller (10) | Walsh Jesuit High School | Cuyahoga Falls | OH |
| 75 | 3:30.32 | Cindy Cheng (12), Erika Price (11), Allie Magrino (12), Emily Kliewer (11) | Dr. Phillips High School | Orlando | FL |
| 76 | 3:30.33 | Jasmine K Mau (12), Kelly Fricke (11), Katherine Woo (9 ), Lia Foster (9 ) | Punahou School | Honolulu | HI |
| 77 | 3:30.35 | Lexus VanHoven (9 ), Emily Lundquist (12), Emliy Converse (10), Gabby Higgins (9 ) | East Grand Rapids High School | Grand Rapids | MI |
| 78 | 3:30.36 | Lily Gasaway (12), Kelleigh Haley (10), Laura Garcia (12), Katie Ledecky (11) | Stone Ridge School | Bethesda | MD |
| 79 | 3:30.46 | Sarah Carter (9 ), Megan Johnson (9 ), Reagan Cook (12), Emily Thrasher (10) | Homestead High School | Fort Wayne | IN |
| 79 | 3:30.46 | Payton Neff (9 ), Ania Gawronska (11), Emily Reese (9 ), Tori Eberwein (12) | The Woodlands High School | The Woodlands | TX |
| 81 | 3:30.50 | Wheeler Jordan (10), Wehrmeister Hannah (9 ), Wehrmeister Sarah (10), Fant Britney (12) | Atascocita High School | Humble | TX |
| 82 | 3:30.56 | Aubrey Jones (12), Emma Fish (10), Joonyoung Lee (9 ), Katherine Aikins (10) | Lambert High School | Suwanee | GA |
| 83 | 3:30.58 | Camille Hui (9 ), Marie-Pierre Delisle (12), Julia Read (10), Sarah Shimomura (11) | Archbishop Mitty High School | San Jose | CA |
| 83 | 3:30.58 | Maddie McNichol (12), Meghan Eife (12), Rachel Hazard (9 ), Abby Kosic (10) | West Chester Henderson High School | West Chester | PA |
| 85 | 3:30.60 | Breinne Thorne (10), Kira Zubar (12), Sarah Coolidge (11), Jennie Novak (9 ) | Eden Prairie High School | Eden Prairie | MN |
| 86 | 3:30.61 | Chanel Bonin (12), Stormy Malarik (12), Deirdre Gerke (12), Carolyn Bischoff (9 ) | South Lyon High School | South Lyon | MI |
| 87 | 3:30.67 | Haley Herzberg (10), Laura Brown (10), Sophia Rodriguez (12), Megan Strickland (12) | Churchill High School | San Antonio | TX |
| 88 | 3:30.72 | Kirsten Jacobsen (10), Mekenna Scheitlin (12), Emma Barnett (11), Andrea Vega (11) | Barrington High School | Barrington | IL |
| 89 | 3:30.77 | Alexis Glunn (10), Anna Lofton (9 ), Kelli Croft (10), Lauren O'Malley (11) | Lassiter High School | Marietta | GA |
| 89 | 3:30.77 | Cecelia Williams (12), Ali Lombardi (11), Morgan Ayers (9 ), Delaney Barnard (12) | Lawton Chiles High School | Tallahassee | FL |
| 91 | 3:30.82 | Abby Stone (11), Keely Lovern (12), Paige Griesse (11), Sydney King (12) | Granville High School | Granville | OH |
| 92 | 3:30.83 | Erica King (11), Grace Kneller (11), Saki Takumiya (11), Loretta Stelnicki (11) | William Fremd High School | Palatine | IL |
| 93 | 3:30.87 | Abby Mack (12), Grace Wydeven (12), Kaitlin Benjamin (12), Amber Zimmerman (11) | Conestoga High School | Berwyn | PA |
| 94 | 3:30.89 | Amber Kovach (11), Summer Svitavsky (10), Heather Svitavsky (10), Claire Doerr (10) | Hawken School | Gates Mills | OH |
| 94 | 3:30.89 | Kayla Epting (11), Lauren Williams (12), Carly Straight (10), Madison Straight (12) | Keller Central High School | Keller | TX |
| 94 | 3:30.89 | Stephanie Garvin (12), Isabelle Bindseil (12), Jordan Morling (10), Katherine Nagler (11) | St. Charles East High School | St. Charles | IL |
| 97 | 3:30.91 | Katie Colwell (10), Alex Aliapoulios (12), Meghan Egan (10), Jenna Egan (12) | New Canaan High School | New Canaan | CT |
| 98 | 3:31.01 | Annie Moosavian (10), Rachel Politi (11), Eleanor Smith (10), Miranda Donley (9 ) | Columbus School For Girls | Columbus | OH |
| 99 | 3:31.02 | Nora Fullenkamp (9 ), Emma King (11), Maddison Eckley (10), Grace Hoffmann (11) | Centerville High School | Centerville | OH |
| 100 | 3:31.18 | Amy Knudsen (11), Emily Vasquez (10), Kaylyn Flatt (11), Morgan Rosas (9 ) | Cherry Creek High School | Greenwood Village | CO |