| Place | Time | Relay Swimmers (Yrs) | School | City | State |
| 1 | 1:40.83 | Hanna House (12), Alex Clarke (11), Veronica Burchill (10), Amy Bilquist (11) | Carmel High School | Carmel | IN |
| 2 | 1:41.45 | Asia Seidt (10), Kennedy Lohman (10), Victoria Horsman (11), Brooke Bauer (10) | Sacred Heart Academy | Louisville | KY |
| 3 | 1:41.79 | Emily Eastin (10), Rachel Taylor (9 ), Ella Eastin (11), Lindsey Engel (12) | Crean Lutheran High School | Irvine | CA |
| 4 | 1:41.96 | Zoe Avestruz (11), Bridgette Grobe (12), Kaia Grobe (11), Kylie Dahlgren (11) | Chanhassen High School | Chanhassen | MN |
| 5 | 1:42.05 | Aly Tetzloff (11), Kelsi Artim (9 ), Hannah Kukurugya (9 ), Racheal Bukowski (12) | Crown Point High School | Crown Point | IN |
| 6 | 1:42.09 | Meaghan Raab (12), Vivian Tafuto (11), Gabi Broschard (12), Colette Robinson (10) | Hershey High School | Hershey | PA |
| 7 | 1:42.10 | Jacquelyn Du (11), Erin Kuhn (10), Kayla Churman (11), Morgan Soulia (11) | North Allegheny Senior High School | Wexford | PA |
| 8 | 1:42.22 | Gabby Sims (12), Gabriele Serniute (11), Maddy Sims (12), Emily Albrecht (11) | Downers Grove North High School | Downers Grove | IL |
| 9 | 1:42.50 | Gianna Garcia (10), Heidi Poppe (12), Abigail Miller (10), Cailey Steffens (11) | San Ramon Valley High School | Danville | CA |
| 10 | 1:42.89 | Maggie Sheean (12), Pauline Kaminski (12), Mimi Schneider (12), Colleen Barron (12) | Fenwick High School | Oak Park | IL |
| 11 | 1:43.02 | Kara Lucenti (12), Ingrid Wall (9 ), Elizabeth Gallagher (12), Amelia Girgenti (12) | New Trier High School | Winnetka | IL |
| 12 | 1:43.25 | Ally Howe (12), Selby Sturzenegger (12), Kayla Holman (10), Kathryn Bower (9 ) | Sacred Heart Preparatory | Atherton | CA |
| 13 | 1:43.41 | Heather Merritt (12), Jessica Merritt (10), Margaret Higgs (9 ), Emily Allen (11) | William A. Hough High School | Cornelius | NC |
| 14 | 1:43.77 | Lia Foster (9 ), Madison Balish (9 ), Jasmine K Mau (12), Roxanna Kiessling (12) | Punahou School | Honolulu | HI |
| 15 | 1:43.81 | Kate Moreton (12), Olivia Anderson (11), Emma Wittmer (11), Rachel Wittmer (9 ) | Edina High School | Edina | MN |
| 16 | 1:43.92 | Alex Cleveland (12), Taite Kitchel (11), Audrey Battisti (11), Gretta DeCoursey (9 ) | Zionsville Community High School | Zionsville | IN |
| 17 | 1:43.97 | Emma Seiberlich (9 ), McKenzie Street (11), Emily Rhodes (12), Sarah Baturka (10) | Episcopal Academy | Newtown Square | PA |
| 18 | 1:44.06 | Nicole Emery (12), Sara Stranick (11), Elsa Welshofer (12), Maria Sheridan (12) | Charlotte Catholic High School | Charlotte | NC |
| 19 | 1:44.11 | Annika Kollevoll (11), Madeline Hoff (11), Chloe Miller (10), Kasey Schmidt (11) | The Bolles School | Jacksonville | FL |
| 20 | 1:44.23 | Alyssa Lewandowski (9 ), Lindsay Adamski (11), Megan Doty (10), Maddie Barta (11) | Arrowhead High School | Hartland | WI |
| 21 | 1:44.29 | Lizzy Baertlein (12), Addison Skogman (11), Kierston Farley-Sepe (10), Kaersten Meitz (12) | Waukesha South High School | Waukesha | WI |
| 21 | 1:44.29 | Carly Quast (9 ), Madison Preiss (10), Alexis Schaaf (9 ), Anna Petty (10) | Wayzata High School | Plymouth | MN |
| 23 | 1:44.31 | Maggie Jahns (10), Abbey Schneider (11), Lizzy Pfeifer (9 ), Lauryn Parrish (9 ) | Fishers High School | Fishers | IN |
| 24 | 1:44.37 | Jordan Morling (10), Shea Hoyt (12), Isabel Herb (10), Katherine Nagler (11) | St. Charles East High School | St. Charles | IL |
| 25 | 1:44.38 | Christina Campbell (9 ), Kirsten Vose (11), Katie Altmayer (12), Kate Herrill (10) | Flintridge Sacred Heart Academy | La Canada | CA |
| 26 | 1:44.45 | Maria Jardeleza (11), Claire Voss (10), Maria Kyle (10), Olivia Andrew (9 ) | Loyola Academy | Wilmette | IL |
| 26 | 1:44.45 | Emma Cleason (9 ), Shannon Cowley (12), Katie Orringer (9 ), Katie Portz (10) | Skyline High School | Ann Arbor | MI |
| 28 | 1:44.52 | Katie Glavinovich (9 ), Catherine Lowengrub (10), Kaitlyn Albertoli (11), Karli Thuen (11) | Jserra Catholic High School | San Juan Capistrano | CA |
| 29 | 1:44.53 | Megan Bestor (12), Ashley Mallon (11), Jane Johnson (9 ), Katherine Aikins (10) | Lambert High School | Suwanee | GA |
| 30 | 1:44.57 | Kassidy Henson (10), Julienne Tadena (11), Heaven Quintana (10), Summer Spradley (11) | Granite Bay High School | Granite Bay | CA |
| 31 | 1:44.65 | Alexandra Rieger (12), Kathleen Benjamin (12), Emma Barksdale (11), Chenoa Devine (10) | Davis Senior High School | Davis | CA |
| 32 | 1:44.67 | Abby Wiet (11), Julia Valentine (12), Rina Laios (10), Kaylee Witkiewicz (11) | Thomas Worthington High School | Worthington | OH |
| 33 | 1:44.77 | Annie Spalding (12), Bethany Galat (12), Kimberly Hochstedler (12), Casey Ziegert (11) | Penn High School | Mishawaka | IN |
| 34 | 1:44.84 | Joyce Lin (9 ), Morganne McKennan (10), Lauren Smith (9 ), Camille Hui (9 ) | Archbishop Mitty High School | San Jose | CA |
| 35 | 1:44.86 | Miriyam Ghali (11), Lindsey Swartz (11), Tyla Martin (11), Marta Ciesla (9 ) | Pine Crest School | Fort Lauderdale | FL |
| 36 | 1:44.92 | Emma Broome (9 ), Taylor Shewchuk (11), Gillian Rieth (10), Emma Segneri (9 ) | Chaparral High School | Scottsdale | AZ |
| 37 | 1:44.93 | Keely Lovern (12), Kassandra Mestemaker (12), Abby Stone (11), Sydney King (12) | Granville High School | Granville | OH |
| 38 | 1:45.00 | Skylar Duggins (10), Caroline Weigand (12), Reagan Cook (12), Katie Krivacs (12) | Homestead High School | Fort Wayne | IN |
| 39 | 1:45.02 | Karley Mattson (11), Molly Washko (12), Gabi D'abato (12), Brooke Delventhal (11) | Strongsville High School | Strongsville | OH |
| 40 | 1:45.22 | Kristina Li (12), Jessica Chen (12), Scarlett Sun (12), Emily Zhang (11) | Thomas S. Wootton High School | Rockville | MD |
| 41 | 1:45.23 | Janet Hu (12), Jade Chen (9 ), Julia Capobianco (11), Elise Bourdelais (10) | Oakton High School | Vienna | VA |
| 42 | 1:45.24 | Alyssa Woltemath (12), Catriona Macgregor (9 ), Victoria Edwards (9 ), Maggie Taylor (9 ) | Westlake High School | Austin | TX |
| 43 | 1:45.36 | Kristen Hong (11), Marie Claire Schillinger (11), Annie Larsen (12), Elise Goetzl (12) | Miramonte High School | Orinda | CA |
| 44 | 1:45.42 | Maddy Cohen (9 ), Erin Scott (10), Devan Sweeney (9 ), Megan Hansen (11) | Brookwood High School | Snellville | GA |
| 45 | 1:45.45 | Pure Maleenont (11), Rebecca Titterton (11), Carly Drew (10), Eva Crouse (9 ) | Suffield Academy | Suffield | CT |
| 45 | 1:45.45 | Allie Parent (11), Brooke Malone (12), Claire Maiocco (9 ), Ellie Wood (12) | Trinity Preparatory School | Winter Park | FL |
| 47 | 1:45.50 | Sara Bentley (12), Sydney Sorenson (12), Grace Snyder (9 ), Katie Sailer (11) | Ames High School | Ames | IA |
| 48 | 1:45.51 | Elliott Schinella (12), Hannah Knoop (12), Ellyse Conn (10), Roxy Griffore (11) | Mercy High School | Farmington Hills | MI |
| 49 | 1:45.53 | Natalie Dowzicky (12), Hayli Randolph (12), Carolyn Yang (12), Meagan Dollard (12) | Germantown Academy | Fort Washington | PA |
| 50 | 1:45.54 | Solie Laughlin (11), Alicia Harrison (9 ), Lindsay Clark (9 ), Paisley O'Shea (11) | Ventura High School | Ventura | CA |
| 51 | 1:45.55 | Laura Norman (12), Kendall Hazleton (11), Ashley Ward (11), Audrey McMurry (9 ) | Keller High School | Keller | TX |
| 52 | 1:45.57 | Ali Galyer (10), Savanna Faulconer (10), Amanda McNulty (12), Lillie Anderson (10) | J.L. Mann High School | Greenville | SC |
| 52 | 1:45.57 | Georgia Brown (12), Madison Straight (12), Lauren Williams (12), Kayla Epting (11) | Keller Central High School | Keller | TX |
| 54 | 1:45.58 | McKenna Avery (12), Caroline Kelly (12), Carly Kramer (10), Nicole Olsen (12) | Plainfield Central Co-op | Plainfield | IL |
| 55 | 1:45.59 | Caroline Baldwin (12), Gwyn Devin (10), Erika Daniel (12), Sarah Cronin (12) | Westfield High School | Westfield | NJ |
| 56 | 1:45.60 | Andrea Laliena (11), Emily Gibson (12), Raquel Remick (12), Camryn Barry (9 ) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 57 | 1:45.61 | Caroline Gaertner (11), Heather Farley (12), Emily Kishish (10), Lorelei Gaertner (9 ) | Visitation High School | Mendota Heights | MN |
| 58 | 1:45.64 | Maureen Sullivan (12), Jordan Decker (10), Ashley Volpenhein (9 ), Julia Volpenhein (11) | Mason High School | Mason | OH |
| 58 | 1:45.64 | Kate Rogers (12), Therese Larson (10), Serena Yan (12), Allison Eppinga (10) | Saline Area Schools | Saline | MI |
| 60 | 1:45.66 | Kamryn Gallowich (11), Sydney Weeks (10), Beth Ryan (11), Erin Stevens (12) | Naperville Central High School | Naperville | IL |
| 61 | 1:45.70 | Tru Walor (10), Martha Haas (9 ), Cecilia Ostergren (10), Abigail Saddler (12) | Minnetonka High School | Minnetonka | MN |
| 62 | 1:45.87 | Elena Adcock (9 ), Basira Knight (12), Megan Owens (12), Christine Kerr (11) | Cary High School | Cary | NC |
| 62 | 1:45.87 | Kate Canfield (11), Alexis Yager (9 ), Annie Gosselin (11), Elaina Ricci (11) | Rosary High School | Aurora | IL |
| 64 | 1:45.90 | Grace Haskett (9 ), Marah Bieger (11), Madeline Wyke (11), Wildemann Karen (11) | Columbus North High School | Columbus | IN |
| 65 | 1:45.93 | Lindsay Painton (10), Sydney Cutler (11), Jennae Frederick (9 ), Amy Lenderink (10) | Regis Jesuit High School | Aurora | CO |
| 66 | 1:45.97 | Sarah Pushis (12), Ciana Heaston (12), Abigail Johnston (9 ), Mykenzie Kostka (10) | Carroll High School | Fort Wayne | IN |
| 66 | 1:45.97 | Lilly Morgan (11), Madeleine Vonderhaar (10), Kat Akin (10), Katie Summe (10) | Notre Dame Academy | Park Hills | KY |
| 68 | 1:46.14 | Bailey Moynihan (12), Katie Wells (11), Diana Capota (11), Connie Chrones (10) | Glenbrook South High School | Glenview | IL |
| 68 | 1:46.14 | Tessa Wilson (12), Katie Lance (11), Jessica Green (10), Heather Kudialis (10) | Science Hill High School | Johnson City | TN |
| 70 | 1:46.16 | Lindsay Temple (12), Emma Kohlenstein (12), Julieyanna Parker (10), Megan Bull (9 ) | Hillsborough High School | Hillsborough | NJ |
| 70 | 1:46.16 | Betsy Trace (12), Jamie Krupp (12), Erin Sheehan (10), Alexandra Hedden (10) | Upper Arlington High School | Upper Arlington | OH |
| 72 | 1:46.18 | Honore Collins (10), Ana Woods (10), Iana Wolff (10), Rosselyn Lopez (12) | Evanston Township High School | Evanston | IL |
| 73 | 1:46.19 | Kara Vandawater (12), Sydney Mcdowell (9 ), Madison Trimble (12), Erin Hudson (11) | Rockford High School | Rockford | MI |
| 74 | 1:46.20 | Olivia DiStefano (11), Erin O'Neill (10), Leah Brown (10), Kailyn Evans (9 ) | North Penn High School | Lansdale | PA |
| 74 | 1:46.20 | Sandra Soe (11), Layna Auchard (12), Lauren Green (9 ), Annalisa Perez (10) | Pioneer High School | San Jose | CA |
| 76 | 1:46.21 | Charity Bennett (10), Gabrielle Mazero (12), Kylie Sutherlin (10), Lauren Blanchard (10) | Hamilton Southeastern High School | Fishers | IN |
| 77 | 1:46.22 | Devin Newton (12), Nora Deleske (9 ), Gabrielle Limon (9 ), Taylor Newton (10) | Edison High School | Huntington Beach | CA |
| 77 | 1:46.22 | Tyler Wickesberg (11), Olivia Heflin (9 ), Elle Rivenbark (10), Kelsey Robison (10) | Eldorado High School | Albuquerque | NM |
| 79 | 1:46.24 | Taylor Garcia (11), Anna Giesler (10), Abi Johns (12), Clara Steeby (10) | Holland High School | Holland | MI |
| 80 | 1:46.26 | Elise Haan (11), Niki Fistrovic (11), Kayla Tennant (9 ), Nora Gillen (11) | Gulf Coast High School | Naples | FL |
| 81 | 1:46.27 | Bailey Sandon (10), Lindsey Horejsi (10), Ahnika Jensen (10), Anna Andersen (10) | Albert Lea High School | Albert Lea | MN |
| 81 | 1:46.27 | Madison Hart (10), Annie O'Brien (12), Emily Wolfe (11), Ilira Zherka (9 ) | Haverford High School | Havertown | PA |
| 83 | 1:46.33 | Julia Box (11), Sidney Andrew (11), Sienna Stonesmith (11), Logan Tidstrom (10) | Fairview High School | Boulder | CO |
| 84 | 1:46.39 | Jennifer Marrkand (12), Emma Gosselin (10), Courteney Martin (12), Alison Kea (9 ) | Westford Academy | Westford | MA |
| 85 | 1:46.43 | Lea Koob (11), Margaux Thompson (10), Amy Knudsen (11), Morgan Rosas (9 ) | Cherry Creek High School | Greenwood Village | CO |
| 86 | 1:46.45 | Emily Gibson (12), Olivia Coffey (11), Elizabeth Nedrow (12), Casey Rose (11) | Southlake Carroll High School | Southlake | TX |
| 87 | 1:46.46 | Maggie Durning (12), Mckenzie Klee (11), Amber Cortazzo (12), Alexandra Rockett (12) | Wilson High School | West Lawn | PA |
| 88 | 1:46.51 | Hanna M. Gresser (9 ), Carly C. Hart (9 ), Lauren E. Heller (10), Alyssa K. Heller (11) | Walsh Jesuit High School | Cuyahoga Falls | OH |
| 89 | 1:46.52 | Rachel Baker (12), Elena Page (9 ), Breinne Thorne (10), Marla Albanese (11) | Eden Prairie High School | Eden Prairie | MN |
| 90 | 1:46.56 | Abby Bittel (12), Breanna Roman (12), Leslie Campbell (11), Melissa Karman (11) | The Baylor School | Chattanooga | TN |
| 91 | 1:46.57 | Caylee Casbon (9 ), Cara Kroger (9 ), Amy Socha (9 ), Marita Banta-Long (10) | Chesterton High School | Chesterton | IN |
| 91 | 1:46.57 | Emily Lacy (11), Devon Litteral (12), Mercedes Martinez (11), Emeline Root (9 ) | Holland West Ottawa High School | Holland | MI |
| 91 | 1:46.57 | Emily Cornell (10), Olivia Tierney (12), Olivia Giampietro (9 ), Julie Dobson (11) | Villa Maria Academy | Malvern | PA |
| 94 | 1:46.58 | Grace Grzybek (10), Loretta Stelnicki (11), Saki Takumiya (11), Grace Kneller (11) | William Fremd High School | Palatine | IL |
| 95 | 1:46.59 | Meredith Robbins (9 ), Macey Neubauer (10), Staci Herchenbach (10), Sophia Lex (12) | Libertyville High School | Libertyville | IL |
| 96 | 1:46.65 | Hannah Grubbs (12), Katherine Erickson (11), Allison Stevens (12), Sarah Rasmussen (12) | Campolindo High School | Moraga | CA |
| 96 | 1:46.65 | Abby Kunze (10), Sophia Bisch (11), Kristin Erf (12), Ava Howard (9 ) | Stillwater Area High School | Stillwater | MN |
| 98 | 1:46.67 | Natalie McGovern (11), Mackenzie Hornstra (11), Wyli Erlechman (9 ), Grace Soltys (12) | Neuqua Valley High School | Naperville | IL |
| 99 | 1:46.68 | Lesley-Ann Knee (9 ), Bailey Kovac (9 ), Bailey Nero (11), Jazmyn Markel (11) | Fossil Ridge High School | Fort Collins | CO |
| 100 | 1:46.69 | Katie Malach (12), Sarah Haerle (10), Christine Jensen (11), Olivia Treski (11) | Castle High School | Newburgh | IN |