| Place | Time | Swimmer (Yr) | School | City | State |
| 1 | :51.54 | Ally Howe (12) | Sacred Heart Preparatory | Atherton | CA |
| 2 | :52.35 | Janet Hu (12) | Oakton High School | Vienna | VA |
| 3 | :52.46 | Clara Smiddy (12) | South Florida Heat | Miami | FL |
| 4 | :52.95 | Taylor Garcia (11) | Holland High School | Holland | MI |
| 5 | :52.96 | Zoe Avestruz (11) | Chanhassen High School | Chanhassen | MN |
| 6 | :52.97 | Claire Adams (10) | Carmel High School | Carmel | IN |
| 7 | :53.08 | Meaghan Raab (12) | Hershey High School | Hershey | PA |
| 8 | :53.19 | Beata Nelson (10) | Verona / Mount Horeb Co-op | Verona | WI |
| 9 | :53.22 | Elise Haan (11) | Gulf Coast High School | Naples | FL |
| 10 | :53.40 | Asia Seidt (10) | Sacred Heart Academy | Louisville | KY |
| 11 | :53.59 | Nora McCullagh (11) | Charlotte Catholic High School | Charlotte | NC |
| 12 | :53.73 | Hannah Stevens (12) | Lexington High School | Lexington | OH |
| 13 | :53.84 | Hannah Whiteley (10) | Springboro High School | Springboro | OH |
| 14 | :53.85 | Samantha Coloma (10) | Carondelet High School | Concord | CA |
| 15 | :53.91 | Jacquelyn Du (11) | North Allegheny Senior High School | Wexford | PA |
| 16 | :53.93 | Gabby Sims (12) | Downers Grove North High School | Downers Grove | IL |
| 17 | :53.96 | Breanna Robinson (11) | Wawasee High School | Syracuse | IN |
| 18 | :54.02 | Marie Chamberlain (12) | Nauset Regional High School | Eastham | MA |
| 19 | :54.04 | Lisa Bratton (12) | Richland High School | Richland | WA |
| 20 | :54.08 | Jennifer Marrkand (12) | Westford Academy | Westford | MA |
| 21 | :54.12 | Hannah Leach (11) | Hellgate High School | Missoula | MT |
| 21 | :54.12 | Kristina Li (12) | Thomas S. Wootton High School | Rockville | MD |
| 23 | :54.16 | Chanel Bonin (12) | South Lyon High School | South Lyon | MI |
| 24 | :54.37 | Emily Eastin (10) | Crean Lutheran High School | Irvine | CA |
| 24 | :54.37 | Haley Hynes (10) | Lee's Summit North High School | Lee's Summit | MO |
| 26 | :54.39 | Lindsey Engel (12) | Crean Lutheran High School | Irvine | CA |
| 27 | :54.44 | Caroline Baldwin (12) | Westfield High School | Westfield | NJ |
| 27 | :54.44 | Laura Norman (12) | Keller High School | Keller | TX |
| 29 | :54.61 | Victoria Edwards (9 ) | Westlake High School | Austin | TX |
| 30 | :54.66 | Marianne Kahmann (10) | Springboro High School | Springboro | OH |
| 31 | :54.71 | Maria Jardeleza (11) | Loyola Academy | Wilmette | IL |
| 32 | :54.77 | Hanna House (12) | Carmel High School | Carmel | IN |
| 33 | :54.79 | Abigail Kochevar (10) | Rock Canyon High School | Highlands Ranch | CO |
| 34 | :54.80 | Madison Myers (11) | Copley High School | Copley | OH |
| 35 | :54.81 | Sara Metzsch (10) | Century High School | Hillsboro | OR |
| 36 | :54.83 | Courtney Mykkanen (10) | Foothill High School | Santa Ana | CA |
| 37 | :54.85 | Christina Paspalas (11) | Northern Valley Regional High School Demarest | Demarest | NJ |
| 38 | :54.86 | Alyssa Marsh (10) | Pine Lake Preparatory | Mooresville | NC |
| 39 | :54.87 | Hannah Lindsey (10) | Winston Churchill High School | Potomac | MD |
| 40 | :54.93 | Destiny Nelson (11) | Prosper High School | Prosper | TX |
| 41 | :54.94 | Zhada Fields (11) | Toledo St. Ursula Academy | Toledo | OH |
| 42 | :54.98 | Amy Bilquist (11) | Carmel High School | Carmel | IN |
| 43 | :55.01 | Geordie Enoch (12) | McDonogh School | Owings Mills | MD |
| 43 | :55.01 | Maggie Miller (11) | Buckhannon Uphsur High School | Buckhannon | WV |
| 45 | :55.06 | Gianna Garcia (10) | San Ramon Valley High School | Danville | CA |
| 46 | :55.07 | Ali Galyer (10) | J.L. Mann High School | Greenville | SC |
| 47 | :55.09 | Chloe Isleta (10) | Presentation High School | San Jose | CA |
| 48 | :55.16 | Kiley Eble (11) | Gilmour Academy | Gates Mills | OH |
| 49 | :55.22 | Megan Bestor (12) | Lambert High School | Suwanee | GA |
| 50 | :55.23 | Abby Stone (11) | Granville High School | Granville | OH |
| 51 | :55.25 | Maggie Jahns (10) | Fishers High School | Fishers | IN |
| 52 | :55.27 | Morgan McCormick (12) | Arvada West High School | Arvada | CO |
| 53 | :55.28 | Cecilia Hake (12) | Faribault High School | Faribault | MN |
| 53 | :55.28 | Kacey Oberlander (10) | Dallastown High School. | Dallastown | PA |
| 55 | :55.29 | Solie Laughlin (11) | Ventura High School | Ventura | CA |
| 56 | :55.30 | Elizabeth Auckley (12) | Bay High School | Bay Village | OH |
| 57 | :55.33 | Katie Grover (12) | Milton High School | Milton | GA |
| 57 | :55.33 | Elise Hart (12) | Cartersville High School | Cartersville | GA |
| 59 | :55.39 | Heidi Katter (11) | Castilleja School | Palo Alto | CA |
| 60 | :55.40 | Emily Cornell (10) | Villa Maria Academy | Malvern | PA |
| 60 | :55.40 | Emily Meilus (9 ) | Herndon High School | Herndon | VA |
| 62 | :55.47 | Grace Haskett (9 ) | Columbus North High School | Columbus | IN |
| 62 | :55.47 | Kyra Sarazen (11) | St. Thomas Aquinas High School | Dover | NH |
| 64 | :55.48 | Hannah Grubbs (12) | Campolindo High School | Moraga | CA |
| 64 | :55.48 | Heather Merritt (12) | William A. Hough High School | Cornelius | NC |
| 66 | :55.50 | Lindsay Morrow (11) | Evergreen High School | Evergreen | CO |
| 67 | :55.53 | Wheeler Jordan (10) | Atascocita High School | Humble | TX |
| 68 | :55.55 | Alexandra Rieger (12) | Davis Senior High School | Davis | CA |
| 69 | :55.56 | Cara Kroeger (9 ) | Chesterton High School | Chesterton | IN |
| 70 | :55.60 | Sammie Burchill (9 ) | Carmel High School | Carmel | IN |
| 70 | :55.60 | Emma Seiberlich (9 ) | Episcopal Academy | Newtown Square | PA |
| 72 | :55.61 | Aja Malone (12) | Allatoona High School | Acworth | GA |
| 73 | :55.62 | Meghan Lavelle (12) | Lane Tech High School | Chicago | IL |
| 74 | :55.63 | Elliott Schinells (12) | Mercy High School | Farmington Hills | MI |
| 75 | :55.69 | Natalie McGovern (11) | Neuqua Valley High School | Naperville | IL |
| 75 | :55.69 | Catherine Wu (11) | Dawson High School | Pearland | TX |
| 77 | :55.70 | Shelly Drozda (11) | Mountain Range High School | Westminster | CO |
| 78 | :55.71 | Morgan McCafferty (11) | Reynoldsburg High School | Reynoldsburg | OH |
| 79 | :55.74 | Erin Earley (9 ) | Hopkins School | New Haven | CT |
| 80 | :55.75 | Natalie Mudd (10) | Hamilton Southeastern High School | Fishers | IN |
| 81 | :55.80 | Nicole Emery (12) | Charlotte Catholic High School | Charlotte | NC |
| 82 | :55.82 | Ines Charles (12) | Bloomfield Hills High School | Bloomfield Hills | MI |
| 82 | :55.82 | Madison Umberger (11) | Portage Central High School | Portage | MI |
| 84 | :55.83 | Marie-Pierre Delisle (12) | Archbishop Mitty High School | San Jose | CA |
| 84 | :55.83 | Alexandra Rockett (12) | Wilson High School | West Lawn | PA |
| 86 | :55.86 | Breinne Thorne (10) | Eden Prairie High School | Eden Prairie | MN |
| 87 | :55.87 | Darby Goodwin (11) | The Pine School | Hobe Sound | FL |
| 87 | :55.87 | Paige Maynard (11) | Vacaville High School | Vacaville | CA |
| 89 | :55.90 | Julia Box (11) | Fairview High School | Boulder | CO |
| 89 | :55.90 | Alex Grimes (11) | Los Gatos High School | Los Gatos | CA |
| 89 | :55.90 | Claire Young (10) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 92 | :55.93 | Aislinn Light (10) | Mater Dei High School | Santa Ana | CA |
| 93 | :55.97 | Remedy Rule (11) | Western Albemarle High School | Crozet | VA |
| 93 | :55.97 | Julia Samson (10) | Shenendehowa High School | Clifton Park | NY |
| 95 | :55.99 | Alicia Diaz (10) | Alexis I. duPont High School | Greenville | DE |
| 96 | :56.01 | Emma Hadley (10) | Horace Greeley High School | Chappaqua | NY |
| 97 | :56.02 | Kierston Farley-Sepe (10) | Waukesha South High School | Waukesha | WI |
| 98 | :56.05 | Sherridon Dressel (10) | Clay High School | Green Cove Springs | FL |
| 99 | :56.06 | Samantha Gowdy (11) | Mt. Vernon High School | Mt. Vernon | IN |
| 99 | :56.06 | Jordan Morling (10) | St. Charles East High School | St. Charles | IL |