| Place | Time | Relay Swimmers (Yrs) | School | City | State |
| 1 | 1:41.17 | Lacey Locke (12), Alex Clarke (10), Ariana Bullard (12), Veronica Burchill (9 ) | Carmel High School | Carmel | IN |
| 2 | 1:41.68 | Kierston Farley-Sepe (9 ), Haley Wickham (12), Mary Schneider (11), Colleen Barron (11) | Fenwick High School | Oak Park | IL |
| 3 | 1:41.76 | Gianna Garcia (9 ), Heidi Poppe (11), Daniella Orlandi (12), Cailey Steffens (10) | San Ramon Valley High School | Danville | CA |
| 4 | 1:42.16 | Hannah Peiffer (12), Breanna Roman (11), Kimberlee John-Williams (12), Kristen Vredeveld (12) | The Baylor School | Chattanooga | TN |
| 5 | 1:42.77 | Tasija Karosas (12), Rachael Bradford-Feldman (10), Megan Moroney (10), Brenna Ruth (11) | Saint Andrew's School | Boca Raton | FL |
| 6 | 1:42.84 | Madison White (12), Kelsey Witkay (9 ), Madelyn Murphy (9 ), Chelsea Chenault (12) | Carondelet High School | Concord | CA |
| 7 | 1:43.26 | Asia Seidt (9 ), Kennedy Lohman (9 ), Victoria Horsman (10), Brooke Bauer (9 ) | Sacred Heart Academy | Louisville | KY |
| 8 | 1:43.27 | Emily Slabe (11), Bridget Blood (12), Sarah Jenkins (12), Temarie Tomley (11) | Ursuline Academy | Cincinnati | OH |
| 9 | 1:43.36 | Madeleine Jardeleza (12), Evan Swenson (12), Maria Jardeleza (10), Grace Tierney (10) | Loyola Academy | Wilmette | IL |
| 10 | 1:43.38 | Jackie Du (10), Gabrielle Borza (12), Maggie Root (10), Arielle Krigger (12) | North Allegheny Senior High School | Wexford | PA |
| 11 | 1:43.65 | Chandler Soapes (12), Ashley Neidigh (12), Salem Peacock (11), Kasey Schmidt (10) | The Bolles Schools | Jacksonville | FL |
| 12 | 1:43.67 | Kara Lucenti (11), Riley Hayward (12), Jessica Sutherland (12), Amelia Girgenti (11) | New Trier High School | Winnetka | IL |
| 13 | 1:43.96 | Katie Christy (10), Jamie Christy (12), Nicole Stafford (12), Erika Redding (12) | Westminster Schools | Atlanta | GA |
| 14 | 1:44.20 | Taylor Garcia (10), Melissa VanderMeulen (12), Abi Johns (11), Clara Steeby (9 ) | Holland High School | Holland | MI |
| 14 | 1:44.20 | Addisynn Bursch (12), Annie Spalding (11), Bethany Galat (11), Jennifer Priebe (12) | Penn High School | Mishawaka | IN |
| 16 | 1:44.22 | Keely Lovern (11), Kassandra Mestemaker (11), Abby Stone (10), Sydney King (11) | Granville High School | Granville | OH |
| 16 | 1:44.22 | Ariana Waitkavicz (12), Carly Deliberty (12), Gabi Broschard (11), Colette Robinson (9 ) | Hershey High School | Hershey | PA |
| 18 | 1:44.26 | Tara Halsted (12), Kathleen Benjamin (11), Emma Barksdale (10), Alex Rieger (11) | Davis Senior High School | Davis | CA |
| 18 | 1:44.26 | Emily Balog (12), Kirsten Vose (10), Katie Altmayer (11), Kate Herrill (9 ) | Flintridge Sacred Heart Academy | La Canada | CA |
| 20 | 1:44.32 | Sandra Soe (10), Layna Auchard (11), Linnea Mack (12), Sierra Painter (10) | Pioneer High School | San Jose | CA |
| 21 | 1:44.37 | Gabby Sims (11), Maddy Sims (11), Daria Wick (10), Emily Albrecht (10) | Downers Grove North High School | Downers Grove | IL |
| 22 | 1:44.38 | Natalie McGovern (10), Megan Childs (12), Gia Dalesandro (12), Riley Schroedter (12) | Neuqua Valley High School | Naperville | IL |
| 23 | 1:44.42 | Alex Cleveland (11), Amanda Short (12), Taite Kitchel (10), Grace Einterz (12) | Zionsville Community High School | Zionsville | IN |
| 24 | 1:44.44 | Taylor Teske (12), Lindsay Adamski (10), Miranda Helbig (12), Mary Warren (11) | Arrowhead High School | Hartland | WI |
| 25 | 1:44.47 | Sarah Baturka (9 ), Rachel Repke (12), Emily Rhodes (11), Tara O'Prey (12) | The Episcopal Academy | Newtown Square | PA |
| 25 | 1:44.47 | Emma Paulson (12), Madison Preiss (9 ), Mackenzie Merriam (12), Anna Petty (9 ) | Wayzata High School | Plymouth | MN |
| 27 | 1:44.87 | Kylie Dahlgren (10), Bridgette Grobe (11), Zoe Avestruz (10), Kaia Grobe (10) | Chanhassen High School | Chanhassen | MN |
| 28 | 1:44.92 | Cheyenne Low (12), Burton Maggie (12), Annie Tran (11), Emma Henderson (12) | University High School | Irvine | CA |
| 29 | 1:44.99 | Kaylee Witkiewicz (10), Maren Reeder (12), Julia Valentine (11), Abby Jank (12) | Thomas Worthington High School | Worthington | OH |
| 30 | 1:45.00 | Kaitlyn Albertoli (10), Karli Thuen (10), Katie McLaughlin (10), Rebecca Millard (12) | Jserra Catholic High School | San Juan Capistrano | CA |
| 31 | 1:45.12 | Colleen VandePoel (12), Chelsea Rish (12), Anna Battistello (12), Caroline Fender (12) | Holland West Ottawa High School | Holland | MI |
| 32 | 1:45.13 | Bailey Pressey (12), Caroline Weigand (11), Reagan Cook (11), Katie Krivacs (11) | Homestead High School | Fort Wayne | IN |
| 33 | 1:45.21 | Kristina Li (11), Jessica Chen (11), Scarlett Sun (11), Emily Zhang (10) | Thomas S. Wootton High School | Rockville | MD |
| 34 | 1:45.28 | Rachel Hoeve (11), Jillena Everest (11), Morgan Bullock (9 ), Leah Ritsema (12) | Zeeland High School | Zeeland | MI |
| 35 | 1:45.31 | Samantha Gowdy (10), Claudia Crawford (9 ), Kelsey Turner (12), Clara Baggett (11) | Mt. Vernon High School | Mt. Vernon | IN |
| 36 | 1:45.37 | Emilee Dinkel (9 ), Sammie Guay (11), Bailey Nero (10), Rhianna Williams (12) | Fossil Ridge High School | Fort Collins | CO |
| 37 | 1:45.45 | Michelle Law (12), Rachel Prorok (12), Anna Williams (12), Amy Novak (12) | Naperville North High School | Naperville | IL |
| 38 | 1:45.47 | Lauren Day (12), Madeline Hazle (12), Leah Pfitzer (12), Katheryn Nolen (12) | Kingwood High School | Kingwood | TX |
| 39 | 1:45.50 | Nicole Zanolli (11), Madeleine Pont (11), Kindle Van Linge (11), Brooke Stenstrom (9 ) | Menlo-Atherton High School | Atherton | CA |
| 39 | 1:45.50 | Jax Shoults (11), Emily Gibson (11), Raquel Remick (11), Audrey Johnstone (10) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 41 | 1:45.52 | Nicole Emery (11), Sara Stranick (10), Elsa Welshofer (11), Maria Sheridan (11) | Charlotte Catholic High School | Charlotte | NC |
| 41 | 1:45.52 | Mckenna Garfien (12), Kelsey Kafka (12), Ariel Jordan (12), Crystal Lore (11) | Torrey Pines High School | San Diego | CA |
| 43 | 1:45.64 | Maddy Frost (12), Cristina Czyrka (12), Emily Lau (12), Kate Rogers (11) | Saline High School | Saline | MI |
| 44 | 1:45.67 | Aly Tetzloff (10), Alex St. Amour (10), Holly Schuster (11), Racheal Bukowski (11) | Crown Point High School | Crown Point | IN |
| 45 | 1:45.72 | Lindsay Temple (11), Rachel Stoddard (12), Julieyanna Parker (9 ), Jacquelynn Parker (12) | Hillsborough High School | Hillsborough | NJ |
| 46 | 1:45.75 | Elli Schinella (11), Maddy Loniewski (11), Ellyse Conn (9 ), Roxanne Griffore (10) | Mercy High School | Farmington Hills | MI |
| 47 | 1:45.76 | Danielle Yoon (12), Briana Burns (11), Alex Buscher (10), Kelly Denkler (12) | Memorial High School | Houston | TX |
| 48 | 1:45.80 | Lauren Fitzgerald (12), Julienne Tadena (10), Hannah Calton (12), Summer Spradley (10) | Granite Bay High School | Granite Bay | CA |
| 49 | 1:45.81 | Emily Cornell (9 ), Olivia Tierney (11), Lauren Law (12), Sarah Hitchens (12) | Villa Maria Academy | Malvern | PA |
| 50 | 1:45.82 | Hannah Grubbs (11), Katie Erickson (10), Allison Stevens (11), Mary Ashby (10) | Campolindo High School | Moraga | CA |
| 51 | 1:45.83 | Claire Young (9 ), Gabrielle DeLoof (11), Jennifer Maiorana (9 ), Catherine DeLoof (10) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 51 | 1:45.83 | Hayden Walker (12), Madeline Wells (12), Madeline Brandt (11), Kathleen Mulligan (12) | Xavier College Preparatory | Phoenix | AZ |
| 53 | 1:45.84 | Chloe Isleta (9 ), Czsarina Isleta (12), Elise Locke (12), Mackenzie Isleta (11) | Presentation High School | San Jose | CA |
| 54 | 1:45.89 | Emily Bauer (11), Katie Roach (12), Sydney Buckley (11), Olivia Gach (12) | Cheyenne Mountain High School | Colorado Springs | CO |
| 55 | 1:45.98 | Karley Mattson (10), Molly Washko (11), Brooke Delventhal (10), Samantha Hargis (12) | Strongsville High School | Strongsville | OH |
| 56 | 1:46.00 | Kandes Soapes (10), McKenna DeBever (11), Emma Devine (9 ), Lydia Ware (12) | Bishop Kenny High School | Jacksonville | FL |
| 57 | 1:46.01 | Madeleine Eden (12), Olivia Anderson (10), Heather Laedtke (12), Amelia Anderson (10) | Edina High School | Edina | MN |
| 58 | 1:46.10 | Georgia Brown (11), Madison Straight (11), Lauren Williams (11), Kayla Epting (10) | Keller Central High School | Keller | TX |
| 59 | 1:46.16 | Ally Howe (11), Selby Sturzenegger (11), Kayla Holman (9 ), Erica Myers (12) | Sacred Heart Preparatory | Atherton | CA |
| 60 | 1:46.17 | Kimberly Graziano (12), Ashleigh Shanley (12), Rebecca Dickey (12), Johanna Buschhaus (12) | Ann Arbor Skyline High School | Ann Arbor | MI |
| 61 | 1:46.24 | Erin Schoenfeld (11), Marie-Claire Schillinger (10), Annie Larsen (11), Elise Goetzl (11) | Miramonte High School | Orinda | CA |
| 62 | 1:46.25 | Pure Maleenont (10), Rebecca Titterton (10), Carly Drew (9 ), Anna Strzempko (12) | Suffield Academy | Suffield | CT |
| 63 | 1:46.26 | Alexandra Rockett (11), Hannah Sauls (12), Amber Cortazzo (11), Laura Travers (12) | Wilson High School | West Lawn | PA |
| 64 | 1:46.27 | Kam Gallowich (10), Taylor Campbell (12), Julia Roller (12), Erin Stevens (11) | Naperville Central High School | Naperville | IL |
| 64 | 1:46.27 | Hannah Mc Dowell (11), Emily Wulff (11), Sydney Lloyd (11), Erin Newman (12) | Oswego East High School | Oswego | IL |
| 66 | 1:46.28 | Kara Vandawater (11), Kennedy Cutler (12), Madison Trimble (11), Erin Hudson (10) | Rockford High School | Rockford | MI |
| 66 | 1:46.28 | Laura Laderoute (9 ), Robin Pinger (9 ), Brie Balsbough (11), Sidney Pinger (12) | West Linn High School | West Linn | OR |
| 68 | 1:46.30 | Emily Gibson (11), MacKenzie Leonard (12), Sydney Wheeler (10), Casey Rose (10) | Southlake Carroll High School | Southlake | TX |
| 69 | 1:46.32 | Morgan Waggoner (9 ), Laura Duncan (12), Haley Fisher (10), Megan Hawk (11) | Fremont Ross High School | Fremont | OH |
| 69 | 1:46.32 | Megan Bestor (11), Ashley Mallon (10), Ellie Sills (12), Katherine Aikins (9 ) | Lambert High School | Suwanee | GA |
| 71 | 1:46.34 | Natalie Dowzicky (11), Madison Visco (10), Carolyn Yang (11), Hayli Randolph (11) | Germantown Academy | Fort Washington | PA |
| 71 | 1:46.34 | Christina Park (12), Erin Oliphant (9 ), Molly Orbon (12), Abigail Rosenberg (10) | Glenbrook North High School | Northbrook | IL |
| 73 | 1:46.39 | Micaela Janco (11), Ali Ramirez (10), Lauren O'Malley (10), Jordan Drake (12) | Lassiter High School | Marietta | GA |
| 74 | 1:46.40 | Betsy Trace (11), Jamie Krupp (11), Emily Annen (12), Erin Sheehan (9 ) | Upper Arlington High School | Upper Arlington | OH |
| 75 | 1:46.57 | Lindsey Engel (11), Ella Eastin (10), Emily Eastin (9 ), Tricia Saylor (11) | Crean Lutheran High School | Irvine | CA |
| 76 | 1:46.66 | Aislinn Light (9 ), Courtney Hanson (9 ), Brittany Kampfer (9 ), Jiana Vargem (12) | Mater Dei High School | Santa Ana | CA |
| 77 | 1:46.71 | Hannah Whiteley (9 ), Marianne Kahmann (9 ), Shelby Tomasiak (9 ), Ashley Whiteley (12) | Springboro High School | Springboro | OH |
| 78 | 1:46.75 | Annelyse Tullier (10), Kate Boyer (10), Meredith Kelly (12), Lauren Murski (11) | Highland Park High School | Dallas | TX |
| 79 | 1:46.76 | Maureen Sullivan (11), Jordan Decker (9 ), Abbey Esler (9 ), Julia Volpenhein (10) | Mason High School | Mason | OH |
| 80 | 1:46.79 | Emma Wyke (12), Marah Bieger (10), Madeline Wyke (10), Rachael Sollman (12) | Columbus North High School | Columbus | IN |
| 81 | 1:46.83 | Rachel Baker (11), Erin Russoniello (10), Bre Thorne (9 ), Sarah Coolidge (10) | Eden Prairie High School | Eden Prairie | MN |
| 81 | 1:46.83 | Alyssa Ruffing (11), Samantha Tower (12), Rachel Barkey (12), Kaitlyn Ruffing (12) | Franklin Regional High School | Murrysville | PA |
| 83 | 1:46.86 | Charlotte Meyer (12), Audrey Gould (12), Halie Kellett (12), Sarah Kannan (11) | Walt Whitman High School | Bethesda | MD |
| 84 | 1:46.96 | Cece Williams (11), Lia Lombardi (12), Alyssa Yambor-Maul (12), Delaney Barnard (11) | Lawton Chiles High School | Tallahassee | FL |
| 85 | 1:46.98 | Dani Bergeson (10), Veronica Herrmann (12), McKenzie Jeske (12), Jarren Fallgatter (12) | Bismarck Century High School | Bismarck | ND |
| 86 | 1:46.99 | Marissa Cominelli (12), Heather Svitavsky (9 ), Kaitlyn Cerne (12), Caroline Vexler (12) | Hawken School | Gates Mills | OH |
| 86 | 1:46.99 | Emma Vertin (9 ), Sophia Nelson (11), Abby Lunzmann (10), Aimee Fischer (11) | Lincoln Southwest High School | Lincoln | NE |
| 88 | 1:47.01 | Madeleine Salesky (10), Madison Prince-Judd (9 ), Isabelle Kitze (11), Tai Hallstein (12) | Marin Academy | San Rafael | CA |
| 89 | 1:47.04 | Courtney Mudd (12), Gabrielle Mazero (11), Kylie Sutherlin (9 ), Caroline Thomas (10) | Hamilton Southeastern High School | Fishers | IN |
| 90 | 1:47.05 | Abby Wilson (10), India Sherman (11), Abby Brown (12), Anya Kone' (10) | Columbus Academy | Gahanna | OH |
| 91 | 1:47.07 | Carter Flynn (11), Alexandria Donahue (10), Emily Clifford (12), Tara McGowan (12) | H.B. Plant High School | Tampa | FL |
| 92 | 1:47.11 | Sandra Wilk (12), Madison Winstead (9 ), Madison Dedman (10), Courtney Younger (10) | Paul Laurence Dunbar High School | Lexington | KY |
| 92 | 1:47.11 | Kristina Domaschofsky (10), Vanessa Duran (12), Hannah Middleton (9 ), Mary Margaret Soderberg (12) | Ronald Reagan High School | San Antonio | TX |
| 94 | 1:47.15 | Amelia Schilling (12), Anna Justinak (9 ), Sophie Levy (9 ), Elizabeth Kane (12) | Minnetonka High School | Minnetonka | MN |
| 95 | 1:47.16 | Steph Boyd (10), Laura Murphy (12), Connie Gan (12), Jillian Saccogna (11) | Hudson High School | Hudson | OH |
| 96 | 1:47.17 | Megan Conrad (12), Amy Hockman (11), Morgan Hill (9 ), Anna Kolanowski (12) | Sherwood High School | Sandy Spring | MD |
| 97 | 1:47.19 | Madeleine Hoopes (12), Kayla Owens (11), Gina DeFrancesco (11), Monica Sowinski (10) | Greater Latrobe High School | Latrobe | PA |
| 98 | 1:47.20 | Maddy Jacumski (10), Dorotka Tou (10), Victoria Butler (11), Dina Rommel (11) | Clarence High School | Clarence | NY |
| 98 | 1:47.20 | Margaret Cooke (12), Natalie Mueller (11), Alexandra Ford (11), Victoria Eberwein (11) | The Woodlands High School | The Woodlands | TX |
| 100 | 1:47.24 | Kelley Garrow (12), Liz Murphy (11), Kristine Rosenberger (12), Kristen Young (10) | Lyons Township High School | Lagrange | IL |